pH-Dependent Membrane Interactions of the Histidine-Rich Cell-Penetrating Peptide LAH4-L1

Intracellular delivery of quantum dots mediated by a histidine- and arginine-rich HR9 cell-penetrating peptide through the direct membrane translocation mechanism.

Functional peptides that transfer biomaterials, such as semiconductor quantum dots (QDs), into cells in biomaterial research have been developed in recent years. Delivery of QDs conjugated with cell-penetrating peptides (CPPs) into cells by the endocytic pathway was problematic in biomedical applications because of lysosomal trapping. Here, we demonstrate that histidine- and arginine-rich CPPs ...

Evaluation of Cell Penetrating Peptide Delivery System on HPV16E7 Expression in Three Types of Cell Line

Background: The poor permeability of the plasma and nuclear membranes to DNA plasmids are two major barriers for the development of these therapeutic molecules. Therefore, success in gene therapy approaches depends on the development of efficient and safe non-viral delivery systems. Objectives: The aim of this study was to investigate the in vitro delivery of plasmid DNA encoding HPV16 E7 gene...

Intracellular Delivery of Histidine and Arginine Rich Cell Penetrating Peptides into HeLa cell Line

Most bioactive macromolecules, such as DNA and RNA, cannot permeate into cells freely from outside the plasma membrane. Cell penetrating peptides are a group of short peptide sequences that are able to transverse the cell membrane for mediating gene into living cells. In this study we demonstrate two cell penetrating peptides 5H-R9-C and 7H-R9-7H-C. These peptides are synthesized by solid phase...

Membrane-mimetic nanocarriers formed by a dipalmitoylated cell-penetrating peptide.

Arginine-rich cell-penetrating peptides.

Arginine-rich cell-penetrating peptides are short cationic peptides capable of traversing the plasma membranes of eukaryotic cells. While successful intracellular delivery of many biologically active macromolecules has been accomplished using these peptides, their mechanisms of cell entry are still under investigation. Recent dialogue has centered on a debate over the roles that direct transloc...